Ticks (Ixodida) are an order of obligate ectoparasites that produce saliva to facilitate blood feeding. This saliva constitutes a venom containing peptides, proteins, and small molecules that inhibit haemostasis and modulate the immune and nervous system of the hosts. Since tick hosts include numerous mammals, many tick venom molecules are highly adapted to modulate mammalian proteins. Some small venom proteins such as evasins are inhibitors of chemokine signalling and may have utility in treating autoimmune disorders. Disulfide-rich peptides such as holocyclotoxins inhibit synaptic transmission by an unknown mechanism, but could have utility in treating neuronal degeneration or epilepsy. However, the composition of venom produced by most species is poorly described, in part due to highly dynamic changes in venom composition over the course of parasitisation. Here, I will discuss recent projects examining the venom toxins of the eastern paralysis tick, Ixodes holocyclus.