Ant venom has evolved to contain a diverse range of toxins for both offensive and defensive purposes. Some ants have evolved unusually painful stings disproportionate to their size. One such ant is the electric ant (Wasmannia auropunctata). Despite only measuring about 1.5 mm in length, it has earned its name for the electric shock-like pain from its sting. This project aims to identify the toxins in electric ant venom that are responsible for this unusual pain and characterise their activity. As a group of peptide toxins that modulate voltage-gated sodium (NaV) channels have been recently found in stinging ants, we hypothesised that similar peptide toxins may be present in electric ant venom. Using a combination of transcriptomic and proteomic methods, we generated a venom profile of peptide toxins in electric ant venom. We then synthesised the putative NaV channel toxins we found and tested them in vivo in mice and in vitro on cells expressing human NaV channels. We found that these peptide toxins can enable activation of NaV channels at more negative potentials, while also inhibiting their inactivation. We also injected these peptides into fruit flies to test for insecticidal activity and observed that they were able to paralyse and kill them. Overall, we have shown that electric ant venom contains peptide toxins capable of both modulating mammalian NaV channels and incapacitating insects. In the future, we hope to understand which parts of these peptides are important for their activity on NaV channels. This will add to our library of compounds we can use to study these channels.