Arachnid venom peptides have emerged as promising environmentally friendly bioinsecticides against a broad range of agricultural pests, including dipteran (Guo et al., 2018), lepidopteran (Wang et al., 2024), and coleopteran species (Oparin et al., 2023). Their characteristics such as insecticidal selectivity, potency, stability, and biodegradability (Windley et al., 2012) make them attractive candidates for applications beyond conventional insect control, including the management of invasive molluscan pests. Theba pisana (Gastropoda: Helicidae), commonly known as the white garden snail, is an invasive terrestrial species characterized by high fecundity, longevity, and rapid population expansion, with infestations causing substantial agricultural losses and damage to harvest machinery (Kosciolek et al., 2024). All field crops are susceptible to T. pisana, especially barley, canola, pulses, and emerging plants (Abdelgalil and Gad, 2023; Kosciolek et al., 2024). In this study, we developed a video-tracking assay to quantify snail locomotor activity and evaluate the effects of arachnid venoms and venom-derived peptides on T. pisana. A total of 107 crude arachnid venoms were screened, leading to the identification of a venom peptide from the tarantula Avicularia sp. that significantly reduced snail movement. The primary sequence of this molluscicidal peptide was determined to be identical with the previously described κ-theraphotoxin-Aa1a from a related tarantula species, which contains a C-terminal amidation and an inhibitor cystine knot (ICK) motif (Ma et al., 2018). These findings suggest that κ-theraphotoxin-Aa1a represents a promising lead candidate for the development of novel peptide-based molluscicides.